Capto s chemical compatibility9/26/2023 ![]() ![]() Ligand coupling method: aldehyde coupling via NH 2 residues of the ligandīinding capacity: 25–35 g IgG/L resin depending on flow rate, column height, and contact timeĮlution conditions: 20 mM citric acid or acetic acid, pH 3–4, or 20 mM sodium acetate, 1. 2 The pressure/flow characteristics describes the relationship between pressure and flow under the set circumstances. Efficient aggregate removal at high load of monoclonal antibodies. High binding capacity, > 100 mg MAb/mL medium. Ligand: CaptureSelect™ FcXL affinity ligand Chemical stability6 Stable to commonly used aqueous buffers 1.0 M NaOH7 8 M urea, 6 M guanidine hydrochloride, 70 ethanol Storage 20 ethanol, 4☌ to 30☌ 1 Median particle size of the cumulative volume distribution. Capto S ImpAct is a strong cation exchange chromatography medium (resin) developed for intermediate purification and polishing of monoclonal antibodies (MAbs) and other biomolecules. Matrix: agarose-based, aldehyde-activated ![]() The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free). Human specific, no binding to bovine antibodies, for exampleĬaptureSelect™ FcXL Affinity Matrix purifies IgGs and Fc fusion proteins directly from complex source materials in a single step with high purity and yield.ĬaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology.Mild elution, making it suitable for Fc fusion proteins.The pressure includes pressure drop from the bed and the column. Capto adhere is a multimodal strong anion exchanger for BioProcess applications. Running conditions: AxiChrom 1000 for Capto S, Chromaflow 1000 for Q Sepharose Fast Flow, 20 cm packed bed, with water at 20✬. It recognizes the CH3 domain of all human IgG subclasses (IgG1, 2, 3, and 4).įeatures of this affinity matrix include: Chemical compatibility See Table 2 Storage 4☌ to ambient temperature Shelf life in 20 EtOH >1 year at ambient temperature Operational temperature 440☌ Autoclaving conditions 0. Pressure/flow curve for Capto S compared to Q Sepharose Fast Flow. CaptureSelect™ FcXL Affinity Matrix has been specifically designed for the purification of recombinant human IgG, Fc fusion proteins, and plasma derived IgG. ![]()
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